› 1t3i

X-ray diffraction
1.8Å resolution

Structure of slr0077/SufS, the Essential Cysteine Desulfurase from Synechocystis PCC 6803

Released:
DOI: 10.2210/pdb1t3i/pdb
PDB entry 1t3i coloured by chain, front view.
PDB entry 1t3i coloured by chain, side view.
PDB entry 1t3i coloured by chain, top view.
Source organism: Synechocystis sp.
Primary publication:
Kinetic and structural characterization of Slr0077/SufS, the essential cysteine desulfurase from Synechocystis sp. PCC 6803.
Tirupati B, Vey JL, Drennan CL, Bollinger JM
Biochemistry 43 12210-9 (2004)
PMID: 15379559

Function and Biology Details

Reaction catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-560592 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Probable cysteine desulfurase Chains: A, B
Molecule details ›
Chains: A, B
Length: 420 amino acids
Theoretical weight: 46.46 KDa
Source organism: Synechocystis sp.
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55793 (Residues: 1-420; Coverage: 100%)
Gene names: csd, slr0077, sufS
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
Carbohydrate polymer : NEW Components: TU4, FRU
Ligand TU4 1 x TU4
Ligand FRU 1 x FRU
1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 88.386Å b: 89.308Å c: 141.815Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.191 0.216
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Fe-S cluster assembly pathways in bacteria.
Ayala-Castro et al. (2008)
9 more

7 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
6 more