› 1uei

X-ray diffraction
2.6Å resolution

Crystal structure of human uridine-cytidine kinase 2 complexed with a feedback-inhibitor, UTP

Released:
DOI: 10.2210/pdb1uei/pdb
PDB entry 1uei coloured by chain, front view.
PDB entry 1uei coloured by chain, side view.
PDB entry 1uei coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for the specificity, catalysis, and regulation of human uridine-cytidine kinase.
Suzuki NN, Koizumi K, Fukushima M, Matsuda A, Inagaki F
Structure 12 751-64 (2004)
PMID: 15130468

Function and Biology Details

Reaction catalysed: 
ATP + cytidine = ADP + CMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-189867 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine-cytidine kinase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9BZX2 (Residues: 1-250; Coverage: 96%)
Gene names: UCK2, UMPK
Sequence domains: Phosphoribulokinase / Uridine kinase family
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand UTP 2 x UTP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: F222
Unit cell:
a: 131.529Å b: 247.748Å c: 91.673Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.245
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Regulation of mammalian nucleotide metabolism and biosynthesis.
Lane et al. (2015)
3 more

5 mentions without citation

NMR structure of the viral peptide linked to the genome (VPg) of poliovirus.
Schein et al. (2006)
4 more