› 1v0l

X-ray diffraction
0.98Å resolution

Xylanase Xyn10A from Streptomyces lividans in complex with xylobio-isofagomine at pH 5.8

Released:
DOI: 10.2210/pdb1v0l/pdb
PDB entry 1v0l coloured by chain, front view.
PDB entry 1v0l coloured by chain, side view.
PDB entry 1v0l coloured by chain, top view.
Source organism: Streptomyces lividans
Primary publication:
Atomic resolution analyses of the binding of xylobiose-derived deoxynojirimycin and isofagomine to xylanase Xyn10A.
Gloster TM, Williams SJ, Roberts S, Tarling CA, Wicki J, Withers SG, Davies GJ
Chem Commun (Camb) 1794-5 (2004)
PMID: 15306887

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150766 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase A Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 34.13 KDa
Source organism: Streptomyces lividans
Expression system: Streptomyces lividans
UniProt:
  • Canonical: P26514 (Residues: 42-354; Coverage: 72%)
Gene name: xlnA
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

2 bound ligands:
Ligand XIF 1 x XIF
Ligand XYP 1 x XYP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 66.018Å b: 46.098Å c: 86.254Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 0.118 0.137
Expression system: Streptomyces lividans

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Citations

1 review citation

High-resolution crystallography and drug design.
Cachau et al. (2005)
6 other articles

3 mentions without citation

Hydration water and bulk water in proteins have distinct properties in radial distributions calculated from 105 atomic resolution crystal structures.
Chen et al. (2008)
2 more