› 1w9s

X-ray diffraction
1.59Å resolution

Structure of a beta-1,3-glucan binding CBM6 from Bacillus halodurans

Released:
DOI: 10.2210/pdb1w9s/pdb
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PDB entry 1w9s coloured by chain, front view.
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PDB entry 1w9s coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Primary publication:
Family 6 carbohydrate binding modules recognize the non-reducing end of beta-1,3-linked glucans by presenting a unique ligand binding surface.
van Bueren AL, Morland C, Gilbert HJ, Boraston AB
J Biol Chem 280 530-7 (2005)
PMID: 15501830

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucan endo-1,3-beta-D-glucosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 142 amino acids
Theoretical weight: 15.67 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KG76 (Residues: 790-925; Coverage: 14%)
Gene name: BH0236
Sequence domains: Carbohydrate binding module (family 6)
Structure domains: Galactose-binding domain-like

Ligands and Environments

2 bound ligands:
Ligand NA 6 x NA
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P1
Unit cell:
a: 30.795Å b: 40.834Å c: 56.118Å
α: 108.95° β: 105.93° γ: 90°
R-values:
R R work R free
0.119 0.117 0.166
Expression system: Escherichia coli BL21(DE3)

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Citations

8 review citations

Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules.
Gilbert et al. (2013)
7 more

4 mentions without citation

Relating destabilizing regions to known functional sites in proteins.
Dessailly et al. (2007)
3 more

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