› 1wu6

X-ray diffraction
1.45Å resolution

Crystal structure of reducing-end-xylose releasing exo-oligoxylanase E70A mutant complexed with xylobiose

Released:
DOI: 10.2210/pdb1wu6/pdb
PDB entry 1wu6 coloured by chain, front view.
PDB entry 1wu6 coloured by chain, side view.
PDB entry 1wu6 coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans C-125
Primary publication:
Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125.
Fushinobu S, Hidaka M, Honda Y, Wakagi T, Shoun H, Kitaoka M
J Biol Chem 280 17180-6 (2005)
PMID: 15718242

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-D-xylose residues from the reducing end of oligosaccharides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191817 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Reducing end xylose-releasing exo-oligoxylanase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 46.07 KDa
Source organism: Alkalihalobacillus halodurans C-125
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KB30 (Residues: 1-388; Coverage: 100%)
Gene name: BH2105
Sequence domains: Glycosyl hydrolases family 8
Structure domains: Glycosyltransferase

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
2 bound ligands:
Ligand NI 1 x NI
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P212121
Unit cell:
a: 53.3Å b: 86.607Å c: 87.711Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.171 0.18
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structural determinants of the substrate specificities of xylanases from different glycoside hydrolase families.
Pollet et al. (2010)
2 more

3 mentions without citation

The Glycoside Hydrolase Family 8 Reducing-End Xylose-Releasing Exo-oligoxylanase Rex8A from Paenibacillus barcinonensis BP-23 Is Active on Branched Xylooligosaccharides.
Valenzuela et al. (2016)
2 more