› 1wup

X-ray diffraction
3Å resolution

Crystal structure of metallo-beta-lactamase IMP-1 mutant (D81E)

Released:
DOI: 10.2210/pdb1wup/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1wup coloured by chain, front view.
PDB entry 1wup coloured by chain, side view.
PDB entry 1wup coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
Probing the role of Asp-120(81) of metallo-beta-lactamase (IMP-1) by site-directed mutagenesis, kinetic studies, and X-ray crystallography.
Yamaguchi Y, Kuroki T, Yasuzawa H, Higashi T, Jin W, Kawanami A, Yamagata Y, Arakawa Y, Goto M, Kurosaki H
J Biol Chem 280 20824-32 (2005)
PMID: 15788415

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase IMP-1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 228 amino acids
Theoretical weight: 25.16 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: P52699 (Residues: 19-246; Coverage: 100%)
Sequence domains: Metallo-beta-lactamase superfamily
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 8 x ZN
Ligand ACY 4 x ACY
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: SPRING-8 BEAMLINE BL40B2
Spacegroup: P1
Unit cell:
a: 49.213Å b: 73.061Å c: 80.89Å
α: 84.81° β: 76.1° γ: 74°
R-values:
R R work R free
0.224 0.224 0.295
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Metallo-β-lactamase structure and function.
Palzkill T. (2013)
7 more

3 mentions without citation

B1-Metallo-β-Lactamases: Where Do We Stand?
Mojica et al. (2016)
2 more