› 1wyz

X-ray diffraction
2.5Å resolution

X-Ray structure of the putative methyltransferase from Bacteroides thetaiotaomicron VPI-5482 at the resolution 2.5 A. Norteast Structural Genomics Consortium target Btr28

Released:
DOI: 10.2210/pdb1wyz/pdb
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PDB entry 1wyz coloured by chain, front view.
PDB entry 1wyz coloured by chain, side view.
PDB entry 1wyz coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Entry authors: Kuzin AP, Chen Y, Forouhar F, Vorobiev SM, Acton T, Ma L-C, Xiao R, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosylmethionine-dependent methytransferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 242 amino acids
Theoretical weight: 27.39 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8A031 (Residues: 1-234; Coverage: 100%)
Gene name: BT_4190
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 85.785Å b: 101.542Å c: 108.802Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.226 0.293
Expression system: Escherichia coli

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