› 1x99

X-ray diffraction
1.4Å resolution

X-ray crystal structure of Xerocomus chrysenteron lectin XCL at 1.4 Angstroms resolution, mutated at Q46M, V54M, L58M

Released:
DOI: 10.2210/pdb1x99/pdb
PDB entry 1x99 coloured by chain, front view.
PDB entry 1x99 coloured by chain, side view.
PDB entry 1x99 coloured by chain, top view.
Source organism: Xerocomellus chrysenteron
Primary publication:
A new lectin family with structure similarity to actinoporins revealed by the crystal structure of Xerocomus chrysenteron lectin XCL.
Birck C, Damian L, Marty-Detraves C, Lougarre A, Schulze-Briese C, Koehl P, Fournier D, Paquereau L, Samama JP
J Mol Biol 344 1409-20 (2004)
PMID: 15561152

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-570772 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lectin Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.27 KDa
Source organism: Xerocomellus chrysenteron
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WZC9 (Residues: 1-143; Coverage: 100%)
Sequence domains: Fungal fruit body lectin
Structure domains: Cytolysin/lectin

Ligands and Environments

2 bound ligands:
Ligand SO4 5 x SO4
Ligand EDO 3 x EDO
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3221
Unit cell:
a: 59.153Å b: 59.153Å c: 149.663Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.174 0.174 0.187
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Disparate proteins use similar architectures to damage membranes.
Anderluh et al. (2008)
7 more

1 mention without citation

Mushroom lectins: specificity, structure and bioactivity relevant to human disease.
Hassan et al. (2015)