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X-ray diffraction
1.84Å resolution

Structure of reduced C73S/C85S putidaredoxin, a [2Fe-2S] ferredoxin from Pseudomonas putida

Released:
DOI: 10.2210/pdb1xlo/pdb
PDB entry 1xlo coloured by chain, front view.
PDB entry 1xlo coloured by chain, side view.
PDB entry 1xlo coloured by chain, top view.
Source organism: Pseudomonas putida
Primary publication:
Redox-dependent structural reorganization in putidaredoxin, a vertebrate-type [2Fe-2S] ferredoxin from Pseudomonas putida.
Sevrioukova IF
J Mol Biol 347 607-21 (2005)
PMID: 15755454

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132340 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putidaredoxin Chains: A, B
Molecule details ›
Chains: A, B
Length: 106 amino acids
Theoretical weight: 11.4 KDa
Source organism: Pseudomonas putida
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00259 (Residues: 2-107; Coverage: 99%)
Gene name: camB
Sequence domains: 2Fe-2S iron-sulfur cluster binding domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

1 bound ligand:
Ligand FES 2 x FES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: C2221
Unit cell:
a: 50.098Å b: 75.097Å c: 108.108Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.23 0.238
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

Structural biology of redox partner interactions in P450cam monooxygenase: a fresh look at an old system.
Sevrioukova et al. (2011)
2 more