1xrk

X-ray diffraction
1.5Å resolution

Crystal structure of a mutant bleomycin binding protein from Streptoalloteichus hindustanus displaying increased thermostability

Released:
Model geometry
Fit model/data
Primary publication:
Engineering a selectable marker for hyperthermophiles.
J Biol Chem 280 11422-31 (2005)
PMID: 15640151

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-148074 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bleomycin resistance protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.92 KDa
Source organism: Streptoalloteichus hindustanus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17493 (Residues: 1-124; Coverage: 100%)
Gene name: ble
Sequence domains: Glyoxalase superfamily protein
Structure domains: 2,3-Dihydroxybiphenyl 1,2-Dioxygenase, domain 1

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P21
Unit cell:
a: 43.983Å b: 66.617Å c: 47.179Å
α: 90° β: 117.43° γ: 90°
R-values:
R R work R free
0.175 0.174 0.194
Expression system: Escherichia coli BL21(DE3)