› 1z5y

X-ray diffraction
1.94Å resolution

Crystal Structure Of The Disulfide-Linked Complex Between The N-Terminal Domain Of The Electron Transfer Catalyst DsbD and The Cytochrome c Biogenesis Protein CcmG

Released:
DOI: 10.2210/pdb1z5y/pdb
PDB entry 1z5y coloured by chain, front view.
PDB entry 1z5y coloured by chain, side view.
PDB entry 1z5y coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structural basis and kinetics of DsbD-dependent cytochrome c maturation.
Stirnimann CU, Rozhkova A, Grauschopf U, Grütter MG, Glockshuber R, Capitani G
Structure 13 985-93 (2005)
PMID: 16004871

Function and Biology Details

Reaction catalysed: 
Protein dithiol + NAD(P)(+) = protein disulfide + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141974 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thiol:disulfide interchange protein DsbD Chain: D
Molecule details ›
Chain: D
Length: 143 amino acids
Theoretical weight: 15.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P36655 (Residues: 20-162; Coverage: 26%)
Gene names: JW5734, b4136, cutA2, cycZ, dipZ, dsbD
Sequence domains: Thiol:disulfide interchange protein DsbD, N-terminal
Structure domains: Thiol:disulfide interchange protein DsbD, N-terminal domain
Thiol:disulfide interchange protein DsbE Chain: E
Molecule details ›
Chain: E
Length: 149 amino acids
Theoretical weight: 17.08 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA86 (Residues: 43-185; Coverage: 77%)
Gene names: JW2183, b2195, ccmG, dsbE, yejQ
Sequence domains: Redoxin
Structure domains: Glutaredoxin

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand EDO 4 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 53.574Å b: 53.165Å c: 63.833Å
α: 90° β: 104.88° γ: 90°
R-values:
R R work R free
0.235 0.235 0.27
Expression system: Escherichia coli

Quick links

Citations

16 review citations

How proteins form disulfide bonds.
Depuydt et al. (2011)
15 more

17 mentions without citation

Protein Machineries Involved in the Attachment of Heme to Cytochrome c: Protein Structures and Molecular Mechanisms.
Travaglini-Allocatelli C. (2013)
16 more