› 1z8s

Solution NMR

DnaB binding domain of DnaG (P16) from Bacillus stearothermophilus (residues 452-597)

Released:
DOI: 10.2210/pdb1z8s/pdb
PDB entry 1z8s coloured by chain, ensemble of 10 models, front view.
PDB entry 1z8s coloured by chain, ensemble of 10 models, side view.
PDB entry 1z8s coloured by chain, ensemble of 10 models, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Solution structure of the helicase-interaction domain of the primase DnaG: a model for helicase activation.
Syson K, Thirlway J, Hounslow AM, Soultanas P, Waltho JP
Structure 13 609-16 (2005)
PMID: 15837199

Function and Biology Details

Reaction catalysed: 
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-194913 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase Chain: A
Molecule details ›
Chain: A
Length: 146 amino acids
Theoretical weight: 16.85 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X4D0 (Residues: 452-597; Coverage: 25%)
Gene name: dnaG
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: torsion angle dynamics followed by cartesian slow-cool annealing energy minimisation
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Mechanisms for initiating cellular DNA replication.
Costa et al. (2013)
6 more

11 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
10 more