Function and Biology Details
Reactions catalysed:
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O(2) = 3-(methylthio)propanoate + formate + CO
1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + O(2) = 4-(methylthio)-2-oxobutanoate + formate
Biochemical function:
Biological process:
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
monomeric (preferred)
Assembly name:
Acireductone dioxygenase (preferred)
PDBe Complex ID:
PDB-CPX-195631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ligands and Environments
1 bound ligand:
No modified residues
Experiments and Validation Details
Refinement method:
combined torsional and cartesian dynamics simulated annealing
with residual dipolar couplings, NOE, chemical shift and dihedral restraints
Expression system: Escherichia coli BL21(DE3)