› 1a8t

X-ray diffraction
2.55Å resolution

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Metallo-beta-lactamase type 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 232 amino acids
Theoretical weight: 25.39 KDa
Source organism: Bacteroides fragilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P25910 (Residues: 18-249; Coverage: 100%)
Gene names: ccrA, cfiA
Structure domains: Ribonuclease Z/Hydroxyacylglutathione hydrolase-like

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand 061 1 x 061
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 71.36Å b: 170.23Å c: 40.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 not available 0.319
Expression system: Escherichia coli

Quick links

Citations

20 review citations

Metallo-beta-lactamases: the quiet before the storm?
Walsh et al. (2005)
19 more

12 mentions without citation

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
11 more