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CARBAMOYL PHOSPHATE SYNTHETASE: CAUGHT IN THE ACT OF GLUTAMINE HYDROLYSIS

Source organism: Escherichia coli
Biochemical function: ligase activity
Biological process: 'de novo' UMP biosynthetic process
Cellular component: carbamoyl-phosphate synthase complex

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EC 6.3.5.5: Carbamoyl-phosphate synthase (glutamine-hydrolyzing)

Reaction catalysed:
(1a) L-glutamine + H(2)O = L-glutamate + NH(3)
Systematic name:
HCO(3)(-):L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating)
Alternative Name(s):
  • CAD (gene name)
  • CPS
  • CarA (gene name)
  • CarB (gene name)
  • Carbamoyl phosphate synthetase
  • Carbamoyl-phosphate synthetase (glutamine-hydrolyzing)
  • Carbamoylphosphate synthetase II
  • Carbamyl phosphate synthetase (glutamine)
  • Carbon-dioxide:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating)
  • GD-CPSase
  • Glutamine-dependent carbamoyl-phosphate synthase
  • Glutamine-dependent carbamyl phosphate synthetase
  • Hydrogen-carbonate:L-glutamine amido-ligase (ADP-forming, carbamate-phosphorylating)

EC 6.3.4.16: Carbamoyl-phosphate synthase (ammonia)

Reaction catalysed:
(1a) ATP + HCO(3)(-) = ADP + carboxyphosphate
Systematic name:
HCO(3)(-):ammonia ligase (ADP-forming, carbamate-phosphorylating)
Alternative Name(s):
  • CPS I
  • CPSI (gene name)
  • Carbamoyl-phosphate synthetase (ammonia)
  • Carbamoyl-phosphate synthetase I
  • Carbamoylphosphate synthase
  • Carbamoylphosphate synthase (ammonia)
  • Carbamoylphosphate synthetase (ammonia)
  • Carbamylphosphate synthetase
  • Carbamylphosphate synthetase I
  • Carbmoylphosphate synthetase
  • Carbon-dioxide--ammonia ligase

GO terms

Sequence families

Pfam Protein families (Pfam)
PF02786
Domain description: Carbamoyl-phosphate synthase L chain, ATP binding domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 8 copies of Pfam domain PF02786 (Carbamoyl-phosphate synthase L chain, ATP binding domain) in Carbamoyl-phosphate synthase large chain. Showing 2 copies in chain A.

PF07722
Domain description: Peptidase C26
Occurring in:
  1. Carbamoyl phosphate synthase small chain

PF00117
Domain description: Glutamine amidotransferase class-I
Occurring in:
  1. Carbamoyl phosphate synthase small chain
The deposited structure of PDB entry 1a9x contains 4 copies of Pfam domain PF00117 (Glutamine amidotransferase class-I) in Carbamoyl-phosphate synthase small chain. Showing 1 copy in chain B.

PF00988
Domain description: Carbamoyl-phosphate synthase small chain, CPSase domain
Occurring in:
  1. Carbamoyl phosphate synthase small chain
The deposited structure of PDB entry 1a9x contains 4 copies of Pfam domain PF00988 (Carbamoyl-phosphate synthase small chain, CPSase domain) in Carbamoyl-phosphate synthase small chain. Showing 1 copy in chain B.

PF02142
Domain description: MGS-like domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 4 copies of Pfam domain PF02142 (MGS-like domain) in Carbamoyl-phosphate synthase large chain. Showing 1 copy in chain A.

PF02787
Domain description: Carbamoyl-phosphate synthetase large chain, oligomerisation domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 4 copies of Pfam domain PF02787 (Carbamoyl-phosphate synthetase large chain, oligomerisation domain) in Carbamoyl-phosphate synthase large chain. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR005483
Domain description: Carbamoyl-phosphate synthase large subunit, CPSase domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
IPR005479
Domain description: Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
IPR011607
Domain description: Methylglyoxal synthase-like domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
IPR006275
Domain description: Carbamoyl-phosphate synthase, large subunit
Occurring in:
  1. Carbamoyl phosphate synthase large chain
IPR029062
Domain description: Class I glutamine amidotransferase-like
Occurring in:
  1. Carbamoyl phosphate synthase small chain
IPR006274
Domain description: Carbamoyl-phosphate synthase, small subunit
Occurring in:
  1. Carbamoyl phosphate synthase small chain
IPR002474
Domain description: Carbamoyl-phosphate synthase small subunit, N-terminal domain
Occurring in:
  1. Carbamoyl phosphate synthase small chain
IPR036480
Domain description: Carbamoyl-phosphate synthase small subunit, N-terminal domain superfamily
Occurring in:
  1. Carbamoyl phosphate synthase small chain
IPR036914
Domain description: Methylglyoxal synthase-like domain superfamily
Occurring in:
  1. Carbamoyl phosphate synthase large chain
IPR005480
Domain description: Carbamoyl-phosphate synthetase, large subunit oligomerisation domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
IPR036897
Domain description: Carbamoyl-phosphate synthetase, large subunit oligomerisation domain superfamily
Occurring in:
  1. Carbamoyl phosphate synthase large chain
IPR011761
Domain description: ATP-grasp fold
Occurring in:
  1. Carbamoyl phosphate synthase large chain
IPR016185
Domain description: Pre-ATP-grasp domain superfamily
Occurring in:
  1. Carbamoyl phosphate synthase large chain
IPR017926
Domain description: Glutamine amidotransferase
Occurring in:
  1. Carbamoyl phosphate synthase small chain
IPR035686
Domain description: Carbamoyl-phosphate synthase small subunit, GATase1 domain
Occurring in:
  1. Carbamoyl phosphate synthase small chain
IPR033937
Domain description: Carbamoyl-phosphate synthase large chain, methylglyoxal synthase-like domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain

Structure domains

CATH CATH domains
3.30.470.20
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: D-amino Acid Aminotransferase; Chain A, domain 1
Homology: ATP-grasp fold, B domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 8 copies of CATH domain 3.30.470.20 (D-amino Acid Aminotransferase; Chain A, domain 1) in Carbamoyl-phosphate synthase large chain. Showing 2 copies in chain A.
3.30.1490.20
Class: Alpha Beta
Architecture: 2-Layer Sandwich
Topology: Dna Ligase; domain 1
Homology: ATP-grasp fold, A domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 8 copies of CATH domain 3.30.1490.20 (Dna Ligase; domain 1) in Carbamoyl-phosphate synthase large chain. Showing 2 copies in chain A.
3.40.50.20
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Rossmann fold
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 8 copies of CATH domain 3.40.50.20 (Rossmann fold) in Carbamoyl-phosphate synthase large chain. Showing 2 copies in chain A.
3.40.50.880
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Rossmann fold
Occurring in:
  1. Carbamoyl phosphate synthase small chain
The deposited structure of PDB entry 1a9x contains 4 copies of CATH domain 3.40.50.880 (Rossmann fold) in Carbamoyl-phosphate synthase small chain. Showing 1 copy in chain B.
3.50.30.20
Class: Alpha Beta
Architecture: 3-Layer(bba) Sandwich
Topology: Glucose Oxidase; domain 1
Homology: Carbamoyl-phosphate synthase small subunit, N-terminal domain
Occurring in:
  1. Carbamoyl phosphate synthase small chain
The deposited structure of PDB entry 1a9x contains 4 copies of CATH domain 3.50.30.20 (Glucose Oxidase; domain 1) in Carbamoyl-phosphate synthase small chain. Showing 1 copy in chain B.
3.40.50.1380
Class: Alpha Beta
Architecture: 3-Layer(aba) Sandwich
Topology: Rossmann fold
Homology: Methylglyoxal synthase-like domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 4 copies of CATH domain 3.40.50.1380 (Rossmann fold) in Carbamoyl-phosphate synthase large chain. Showing 1 copy in chain A.
1.10.1030.10
Class: Mainly Alpha
Architecture: Orthogonal Bundle
Topology: Carbamoyl Phosphate Synthetase; Chain A, domain 4
Homology: Carbamoyl-phosphate synthetase, large subunit oligomerisation domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 4 copies of CATH domain 1.10.1030.10 (Carbamoyl Phosphate Synthetase; Chain A, domain 4) in Carbamoyl-phosphate synthase large chain. Showing 1 copy in chain A.
SCOP SCOP annotations
56067
Class: Alpha and beta proteins (a+b)
Fold: ATP-grasp
Superfamily: Glutathione synthetase ATP-binding domain-like
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 8 copies of SCOP domain 56067 (BC ATP-binding domain-like) in Carbamoyl-phosphate synthase large chain. Showing 2 copies in chain A.
52441
Class: Alpha and beta proteins (a/b)
Fold: PreATP-grasp domain
Superfamily: PreATP-grasp domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 8 copies of SCOP domain 52441 (BC N-terminal domain-like) in Carbamoyl-phosphate synthase large chain. Showing 2 copies in chain A.
52318
Class: Alpha and beta proteins (a/b)
Fold: Flavodoxin-like
Superfamily: Class I glutamine amidotransferase-like
Occurring in:
  1. Carbamoyl phosphate synthase small chain
The deposited structure of PDB entry 1a9x contains 4 copies of SCOP domain 52318 (Class I glutamine amidotransferases (GAT)) in Carbamoyl-phosphate synthase small chain. Showing 1 copy in chain B.
52022
Class: Alpha and beta proteins (a/b)
Fold: The "swivelling" beta/beta/alpha domain
Superfamily: Carbamoyl phosphate synthetase, small subunit N-terminal domain
Occurring in:
  1. Carbamoyl phosphate synthase small chain
The deposited structure of PDB entry 1a9x contains 4 copies of SCOP domain 52022 (Carbamoyl phosphate synthetase, small subunit N-terminal domain) in Carbamoyl-phosphate synthase small chain. Showing 1 copy in chain B.
52336
Class: Alpha and beta proteins (a/b)
Fold: Methylglyoxal synthase-like
Superfamily: Methylglyoxal synthase-like
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 4 copies of SCOP domain 52336 (Carbamoyl phosphate synthetase, large subunit allosteric, C-terminal domain) in Carbamoyl-phosphate synthase large chain. Showing 1 copy in chain A.
48109
Class: All alpha proteins
Fold: Carbamoyl phosphate synthetase, large subunit connection domain
Superfamily: Carbamoyl phosphate synthetase, large subunit connection domain
Occurring in:
  1. Carbamoyl phosphate synthase large chain
The deposited structure of PDB entry 1a9x contains 4 copies of SCOP domain 48109 (Carbamoyl phosphate synthetase, large subunit connection domain) in Carbamoyl-phosphate synthase large chain. Showing 1 copy in chain A.

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