› 1afk

X-ray diffraction
1.7Å resolution

CRYSTAL STRUCTURE OF RIBONUCLEASE A IN COMPLEX WITH 5'-DIPHOSPHOADENOSINE-3'-PHOSPHATE

Released:
DOI: 10.2210/pdb1afk/pdb
PDB entry 1afk coloured by chain, front view.
PDB entry 1afk coloured by chain, side view.
PDB entry 1afk coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crystal structures of ribonuclease A complexes with 5'-diphosphoadenosine 3'-phosphate and 5'-diphosphoadenosine 2'-phosphate at 1.7 A resolution.
Leonidas DD, Shapiro R, Irons LI, Russo N, Acharya KR
Biochemistry 36 5578-88 (1997)
PMID: 9154942

Function and Biology Details

Reaction catalysed: 
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158390 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chains: A, B
Molecule details ›
Chains: A, B
Length: 124 amino acids
Theoretical weight: 13.71 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

1 bound ligand:
Ligand PAP 2 x PAP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X31
Spacegroup: C2
Unit cell:
a: 101.54Å b: 33.36Å c: 73.25Å
α: 90° β: 90.09° γ: 90°
R-values:
R R work R free
0.211 0.211 0.266

Quick links

Citations

39 citation in other articles

Discriminating between homodimeric and monomeric proteins in the crystalline state.
Ponstingl et al. (2000)
38 more

16 mentions without citation

Hypervalent nonbonded interactions of a divalent sulfur atom. Implications in protein architecture and the functions.
Iwaoka et al. (2012)
15 more