› 1akl

X-ray diffraction
2Å resolution

ALKALINE PROTEASE FROM PSEUDOMONAS AERUGINOSA IFO3080

Released:
DOI: 10.2210/pdb1akl/pdb
Model geometry
Fit model/data
Data not deposited
PDB entry 1akl coloured by chain, front view.
PDB entry 1akl coloured by chain, side view.
PDB entry 1akl coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structure of the unliganded alkaline protease from Pseudomonas aeruginosa IFO3080 and its conformational changes on ligand binding.
Miyatake H, Hata Y, Fujii T, Hamada K, Morihara K, Katsube Y
J Biochem 118 474-9 (1995)
PMID: 8690704

Function and Biology Details

Reaction catalysed: 
Preferential cleavage of bonds with hydrophobic residues in P1'.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169815 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serralysin Chain: A
Molecule details ›
Chain: A
Length: 470 amino acids
Theoretical weight: 49.53 KDa
Source organism: Pseudomonas aeruginosa
UniProt:
  • Canonical: Q03023 (Residues: 10-479; Coverage: 98%)
Gene names: PA1249, aprA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 1 x ZN
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 77.16Å b: 176.69Å c: 51.12Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.225

Quick links

Citations

3 review citations

Structural aspects of the metzincin clan of metalloendopeptidases.
Gomis-Rüth FX. (2003)
2 more

13 mentions without citation

Structure-Function Relationships of the Repeat Domains of RTX Toxins.
Baumann U. (2019)
12 more