› 1as0

X-ray diffraction
2Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145470 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chain: A
Molecule details ›
Chain: A
Length: 353 amino acids
Theoretical weight: 40.31 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P10824 (Residues: 2-354; Coverage: 100%)
Gene names: Gnai-1, Gnai1
Sequence domains: G-protein alpha subunit
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand MG 1 x MG
Ligand SO4 1 x SO4
Ligand GSP 1 x GSP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P3221
Unit cell:
a: 78.95Å b: 78.95Å c: 105.29Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins.
Ross et al. (2000)
8 more

10 mentions without citation

Evaluating caveolin interactions: do proteins interact with the caveolin scaffolding domain through a widespread aromatic residue-rich motif?
Byrne et al. (2012)
9 more