› 1asp

X-ray diffraction
2.59Å resolution

X-RAY STRUCTURES AND MECHANISTIC IMPLICATIONS OF THREE FUNCTIONAL DERIVATIVES OF ASCORBATE OXIDASE FROM ZUCCHINI: REDUCED-, PEROXIDE-, AND AZIDE-FORMS

Released:
DOI: 10.2210/pdb1asp/pdb
PDB entry 1asp coloured by chain, front view.
PDB entry 1asp coloured by chain, side view.
PDB entry 1asp coloured by chain, top view.
Source organism: Cucurbita pepo var. melopepo
Primary publication:
X-ray structures and mechanistic implications of three functional derivatives of ascorbate oxidase from zucchini. Reduced, peroxide and azide forms.
Messerschmidt A, Luecke H, Huber R
J Mol Biol 230 997-1014 (1993)
PMID: 8478945

Function and Biology Details

Reaction catalysed: 
4 L-ascorbate + O(2) = 4 monodehydroascorbate + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-153341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-ascorbate oxidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 552 amino acids
Theoretical weight: 61.77 KDa
Source organism: Cucurbita pepo var. melopepo
Expression system: Not provided
UniProt:
  • Canonical: P37064 (Residues: 1-552; Coverage: 100%)
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

4 bound ligands:
Ligand NAG 2 x NAG
Ligand CU 9 x CU
Ligand OH 2 x OH
Ligand PEO 2 x PEO
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 106.33Å b: 105.32Å c: 112.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.16 not available
Expression system: Not provided

Quick links

Citations

15 review citations

Enzymes and associated electron transport systems that catalyse the respiratory reduction of nitrogen oxides and oxyanions.
Berks et al. (1995)
14 more

10 mentions without citation

How Zinc-Binding Systems, Expressed by Human Pathogens, Acquire Zinc from the Colonized Host Environment: A Critical Review on Zincophores.
Bellotti et al. (2021)
9 more