› 1bcr

X-ray diffraction
2.5Å resolution

COMPLEX OF THE WHEAT SERINE CARBOXYPEPTIDASE, CPDW-II, WITH THE MICROBIAL PEPTIDE ALDEHYDE INHIBITOR, ANTIPAIN, AND ARGININE AT ROOM TEMPERATURE

Released:
DOI: 10.2210/pdb1bcr/pdb
PDB entry 1bcr coloured by chain, front view.
PDB entry 1bcr coloured by chain, side view.
PDB entry 1bcr coloured by chain, top view.
Source organisms:
Primary publication:
Peptide aldehyde complexes with wheat serine carboxypeptidase II: implications for the catalytic mechanism and substrate specificity.
Bullock TL, Breddam K, Remington SJ
J Mol Biol 255 714-25 (1996)
PMID: 8636973

Function and Biology Details

Reaction catalysed: 
Preferential release of a C-terminal arginine or lysine residue.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140387 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Serine carboxypeptidase 2 chain A Chain: A
Molecule details ›
Chain: A
Length: 263 amino acids
Theoretical weight: 29.18 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P08819 (Residues: 1-263; Coverage: 59%)
Gene name: CBP2
Sequence domains: Serine carboxypeptidase
Structure domains: alpha/beta hydrolase
Serine carboxypeptidase 2 chain B Chain: B
Molecule details ›
Chain: B
Length: 160 amino acids
Theoretical weight: 18 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P08819 (Residues: 285-444; Coverage: 36%)
Gene name: CBP2
Sequence domains: Serine carboxypeptidase
Structure domains:
ANTIPAIN Chain: C
Molecule details ›
Chain: C
Length: 4 amino acids
Theoretical weight: 608 Da
Source organism: Actinomycetes
Expression system: Not provided

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 1 x NAG
Ligand ARG 1 x ARG
1 modified residue:
Ligand FC0 1 x FC0

Experiments and Validation Details

Entry percentile scores
Spacegroup: P41212
Unit cell:
a: 98.4Å b: 98.4Å c: 209.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 not available not available
Expression system: Not provided

Quick links

Citations

2 review citations

Of barn owls and bankers: a lush variety of alpha/beta hydrolases.
Heikinheimo et al. (1999)
1 more

5 mentions without citation

Glycan Reader: automated sugar identification and simulation preparation for carbohydrates and glycoproteins.
Jo et al. (2011)
4 more