› 1bd0

X-ray diffraction
1.6Å resolution

ALANINE RACEMASE COMPLEXED WITH ALANINE PHOSPHONATE

Released:
DOI: 10.2210/pdb1bd0/pdb
PDB entry 1bd0 coloured by chain, front view.
PDB entry 1bd0 coloured by chain, side view.
PDB entry 1bd0 coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Primary publication:
Reaction of alanine racemase with 1-aminoethylphosphonic acid forms a stable external aldimine.
Stamper GF, Morollo AA, Ringe D, Stamper CG
Biochemistry 37 10438-45 (1998)
PMID: 9671513

Function and Biology Details

Reaction catalysed: 
L-alanine = D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alanine racemase Chains: A, B
Molecule details ›
Chains: A, B
Length: 388 amino acids
Theoretical weight: 43.57 KDa
Source organism: Geobacillus stearothermophilus
UniProt:
  • Canonical: P10724 (Residues: 1-388; Coverage: 100%)
Gene names: alr, dal
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand IN5 2 x IN5
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 98.227Å b: 87.69Å c: 85.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.24 0.24 0.266

Quick links

Citations

8 review citations

Pyridoxal phosphate enzymes: mechanistic, structural, and evolutionary considerations.
Eliot et al. (2004)
7 more

12 mentions without citation

HotPatch: a statistical approach to finding biologically relevant features on protein surfaces.
Pettit et al. (2007)
11 more