› 1bda

X-ray diffraction
3.35Å resolution

CATALYTIC DOMAIN OF HUMAN SINGLE CHAIN TISSUE PLASMINOGEN ACTIVATOR IN COMPLEX WITH DANSYL-EGR-CMK (DANSYL-GLU-GLY-ARG CHLOROMETHYL KETONE)

Released:
DOI: 10.2210/pdb1bda/pdb
PDB entry 1bda coloured by chain, front view.
PDB entry 1bda coloured by chain, side view.
PDB entry 1bda coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Lysine 156 promotes the anomalous proenzyme activity of tPA: X-ray crystal structure of single-chain human tPA.
Renatus M, Engh RA, Stubbs MT, Huber R, Fischer S, Kohnert U, Bode W
EMBO J 16 4797-805 (1997)
PMID: 9305622

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133299 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tissue-type plasminogen activator chain A Chains: A, B
Molecule details ›
Chains: A, B
Length: 265 amino acids
Theoretical weight: 29.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P00750 (Residues: 298-562; Coverage: 49%)
Gene name: PLAT
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand 2Z0 2 x 2Z0
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 43.99Å b: 80.78Å c: 159.16Å
α: 90° β: 90° γ: 90°
Expression system: Escherichia coli

Quick links

Citations

16 review citations

The protein structures that shape caspase activity, specificity, activation and inhibition.
Fuentes-Prior et al. (2004)
15 more

13 mentions without citation

The plasmin-antiplasmin system: structural and functional aspects.
Schaller et al. (2011)
12 more