› 1bls

X-ray diffraction
2.3Å resolution

CRYSTALLOGRAPHIC STRUCTURE OF A PHOSPHONATE DERIVATIVE OF THE ENTEROBACTER CLOACAE P99 CEPHALOSPORINASE: MECHANISTIC INTERPRETATION OF A BETA-LACTAMASE TRANSITION STATE ANALOG

Released:
DOI: 10.2210/pdb1bls/pdb
PDB entry 1bls coloured by chain, front view.
PDB entry 1bls coloured by chain, side view.
PDB entry 1bls coloured by chain, top view.
Source organism: Enterobacter cloacae
Primary publication:
Crystallographic structure of a phosphonate derivative of the Enterobacter cloacae P99 cephalosporinase: mechanistic interpretation of a beta-lactamase transition-state analog.
Lobkovsky E, Billings EM, Moews PC, Rahil J, Pratt RF, Knox JR
Biochemistry 33 6762-72 (1994)
PMID: 8204611

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138639 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 361 amino acids
Theoretical weight: 39.27 KDa
Source organism: Enterobacter cloacae
Expression system: Not provided
UniProt:
  • Canonical: P05364 (Residues: 21-381; Coverage: 100%)
Gene name: ampC
Sequence domains: Beta-lactamase
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

1 bound ligand:
Ligand IPP 2 x IPP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21
Unit cell:
a: 46.5Å b: 83.47Å c: 95.46Å
α: 90° β: 90.01° γ: 90°
R-values:
R R work R free
0.192 not available not available
Expression system: Not provided

Quick links

Citations

12 review citations

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
11 more

7 mentions without citation

Class C β-Lactamases: Molecular Characteristics.
Philippon et al. (2022)
6 more