› 1bo6

X-ray diffraction
2.1Å resolution

ESTROGEN SULFOTRANSFERASE WITH INACTIVE COFACTOR PAP AND VANADATE

Released:
DOI: 10.2210/pdb1bo6/pdb
PDB entry 1bo6 coloured by chain, front view.
PDB entry 1bo6 coloured by chain, side view.
PDB entry 1bo6 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
The sulfuryl transfer mechanism. Crystal structure of a vanadate complex of estrogen sulfotransferase and mutational analysis.
Kakuta Y, Petrotchenko EV, Pedersen LC, Negishi M
J Biol Chem 273 27325-30 (1998)
PMID: 9765259

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-546421 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1E1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 297 amino acids
Theoretical weight: 35.78 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P49891 (Residues: 1-295; Coverage: 100%)
Gene names: Est, Ste, Sult1e1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
Ligand VO4 2 x VO4
Ligand A3P 2 x A3P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P21212
Unit cell:
a: 96.292Å b: 78.909Å c: 80.362Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.207 0.249
Expression system: Escherichia coli

Quick links

Citations

18 review citations

Human sulfotransferases and their role in chemical metabolism.
Gamage et al. (2006)
17 more

5 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
4 more