› 1boi

X-ray diffraction
2.2Å resolution

Function and Biology Details

Reaction catalysed: 
Thiosulfate + cyanide = sulfite + thiocyanate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132741 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiosulfate sulfurtransferase Chain: A
Molecule details ›
Chain: A
Length: 296 amino acids
Theoretical weight: 33.24 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00586 (Residues: 2-297; Coverage: 100%)
Gene name: TST
Sequence domains: Rhodanese-like domain
Structure domains: Rhodanese-like domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CSS 1 x CSS

Experiments and Validation Details

Entry percentile scores
X-ray source: MACSCIENCE M18X
Spacegroup: P21212
Unit cell:
a: 90.69Å b: 73.25Å c: 39.67Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.162 0.219
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Hydrogen Sulfide Biochemistry and Interplay with Other Gaseous Mediators in Mammalian Physiology.
Giuffrè et al. (2018)
1 more

3 mentions without citation

Residues in substrate proteins that interact with GroEL in the capture process are buried in the native state.
Stan et al. (2006)
2 more