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Conformational changes in cubic insulin crystals in the pH range 7-11.

Gursky O, Badger J, Li Y, Caspar DL
Biophys J 63 1210-20 (1992)
Related entries: 1aph, 1cph, 1dph

Cited: 38 times
EuropePMC logo PMID: 1477273

Abstract

To determine the effect of variations in the charge distribution on the conformation of a protein molecule, we have solved the structures of bovine cubic insulin over a pH range from 7 to 11 in 0.1 M and 1 M sodium salt solutions. The x-ray data were collected beyond 2-A resolution and the R factors for the refined models ranged from 0.16 to 0.20. Whereas the positions of most protein and well-ordered solvent atoms are conserved, about 30% of residues alter their predominant conformation as the pH is changed. Conformational switching of A5 Gln and B10 His correlates with the pH dependence of monovalent cation binding to insulin in cubic crystals. Shifts in the relative positions of the A chain NH2-terminal and B chain COOH-terminal groups are probably due to titration of the A1 alpha-amino group. Two alternative positions of B25 Phe and A21 Asn observed in cubic insulin at pH 11 are similar to those found in two independent molecules of the 2Zn insulin dimer at pH 6.4. The conformational changes of the insulin amino acids appear to be only loosely coupled at distant protein sites. Shifts in the equilibrium between distinct conformational substates as the charge distribution on the protein is altered are analogous to the electrostatically triggered movements that occur in many functional protein reactions.

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  4. In Quest for Improved Drugs against Diabetes: The Added Value of X-ray Powder Diffraction Methods. Karavassili F, Valmas A, Fili S, Georgiou CD, Margiolaki I. Biomolecules 7 (2017)
  5. A thing of beauty: Structure and function of insulin's "aromatic triplet". Weiss MA, Lawrence MC. Diabetes Obes Metab 20 Suppl 2 51-63 (2018)

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  2. Traveling-wave ion mobility mass spectrometry of protein complexes: accurate calibrated collision cross-sections of human insulin oligomers. Salbo R, Bush MF, Naver H, Campuzano I, Robinson CV, Pettersson I, Jørgensen TJ, Haselmann KF. Rapid Commun. Mass Spectrom. 26 1181-1193 (2012)
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  5. Strategy for selective chemical cross-linking of tyrosine and lysine residues. Leavell MD, Novak P, Behrens CR, Schoeniger JS, Kruppa GH. J. Am. Soc. Mass Spectrom. 15 1604-1611 (2004)
  6. A tractable genotype-phenotype map modelling the self-assembly of protein quaternary structure. Greenbury SF, Johnston IG, Louis AA, Ahnert SE. J R Soc Interface 11 20140249 (2014)
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  8. Protein purification with vapor-phase carbon dioxide. Winters MA, Frankel DZ, Debenedetti PG, Carey J, Devaney M, Przybycien TM. Biotechnol. Bioeng. 62 247-258 (1999)
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  10. Structure of cubic insulin crystals in glucose solutions. Yu B, Caspar DL. Biophys. J. 74 616-622 (1998)
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  16. Structure and selectivity of a monovalent cation binding site in cubic insulin crystals. Badger J, Kapulsky A, Gursky O, Bhyravbhatla B, Caspar DL. Biophys. J. 66 286-292 (1994)
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  20. Substitution of an Internal Disulfide Bridge with a Diselenide Enhances both Foldability and Stability of Human Insulin. Weil-Ktorza O, Rege N, Lansky S, Shalev DE, Shoham G, Weiss MA, Metanis N. Chemistry 25 8513-8521 (2019)
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  22. Display and interpretation of solvent electron density distributions in insulin crystals. Badger J. J Mol Graph 11 218-21, 233 (1993)
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Related citations provided by authors (5)

  1. Monovalent Cation Binding in Cubic Insulin Crystals. Gursky O, Li Y, Badger J, Caspar DLD Biophys. J. 61 604- (1992)
  2. Flexibility in Crystalline Insulins. Badger J Biophys. J. 61 816- (1992)
  3. Structure of the Pig Insulin Dimer in the Cubic Crystal. Badger J, Harris MR, Reynolds CD, Evans AC, Dodson EJ, Dodson GG, North ACT Acta Crystallogr., B 47 127- (1991)
  4. Water Structure in Cubic Insulin Crystals. Badger J, Caspar DLD Proc. Natl. Acad. Sci. U.S.A. 88 622- (1991)
  5. Zinc-free cubic pig insulin: crystallization and structure determination.. Dodson EJ, Dodson GG, Lewitova A, Sabesan M J Mol Biol 125 387-96 (1978)

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