› 1bsl

X-ray diffraction
1.95Å resolution

STRUCTURE OF ALKANAL MONOOXYGENASE BETA CHAIN

Released:
DOI: 10.2210/pdb1bsl/pdb
PDB entry 1bsl coloured by chain, front view.
PDB entry 1bsl coloured by chain, side view.
PDB entry 1bsl coloured by chain, top view.
Source organism: Vibrio harveyi
Primary publication:
Structure of the beta 2 homodimer of bacterial luciferase from Vibrio harveyi: X-ray analysis of a kinetic protein folding trap.
Thoden JB, Holden HM, Fisher AJ, Sinclair JF, Wesenberg G, Baldwin TO, Rayment I
Protein Sci 6 13-23 (1997)
PMID: 9007973

Function and Biology Details

Reaction catalysed: 
A long-chain aldehyde + FMNH(2) + O(2) = a long-chain fatty acid + FMN + H(2)O + light
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139737 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alkanal monooxygenase beta chain Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 36.38 KDa
Source organism: Vibrio harveyi
Expression system: Escherichia coli
UniProt:
  • Canonical: P07739 (Residues: 1-324; Coverage: 100%)
Gene name: luxB
Sequence domains: Luciferase-like monooxygenase
Structure domains: Luciferase-like domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 58.8Å b: 62Å c: 218.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 not available
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Reduced flavin: donor and acceptor enzymes and mechanisms of channeling.
Tu SC. (2001)
13 other articles

2 mentions without citation

Incorporating dipolar solvents with variable density in Poisson-Boltzmann electrostatics.
Azuara et al. (2008)
1 more