› 1btn

X-ray diffraction
2Å resolution

STRUCTURE OF THE BINDING SITE FOR INOSITOL PHOSPHATES IN A PH DOMAIN

Released:
DOI: 10.2210/pdb1btn/pdb
PDB entry 1btn coloured by chain, front view.
PDB entry 1btn coloured by chain, side view.
PDB entry 1btn coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structure of the binding site for inositol phosphates in a PH domain.
Hyvönen M, Macias MJ, Nilges M, Oschkinat H, Saraste M, Wilmanns M
EMBO J 14 4676-85 (1995)
PMID: 7588597

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178928 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Spectrin beta chain, non-erythrocytic 1 Chain: A
Molecule details ›
Chain: A
Length: 106 amino acids
Theoretical weight: 12.29 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q62261 (Residues: 2199-2304; Coverage: 5%)
Gene names: Elf, Spnb-2, Spnb2, Sptb2, Sptbn1
Sequence domains: Pleckstrin homology domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:
Ligand I3P 1 x I3P
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P4212
Unit cell:
a: 69Å b: 69Å c: 50.8Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.286
Expression system: Escherichia coli BL21

Quick links

Citations

57 review citations

Synthesis and function of 3-phosphorylated inositol lipids.
Vanhaesebroeck et al. (2001)
56 more

30 mentions without citation

Translation of the phosphoinositide code by PI effectors.
Kutateladze TG. (2010)
29 more