› 1cj0

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF RABBIT CYTOSOLIC SERINE HYDROXYMETHYLTRANSFERASE AT 2.8 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb1cj0/pdb
PDB entry 1cj0 coloured by chain, front view.
PDB entry 1cj0 coloured by chain, side view.
PDB entry 1cj0 coloured by chain, top view.
Source organism: Oryctolagus cuniculus
Primary publication:
Crystal structure of rabbit cytosolic serine hydroxymethyltransferase at 2.8 A resolution: mechanistic implications.
Scarsdale JN, Kazanina G, Radaev S, Schirch V, Wright HT
Biochemistry 38 8347-58 (1999)
PMID: 10387080

Function and Biology Details

Reaction catalysed: 
5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine hydroxymethyltransferase, cytosolic Chains: A, B
Molecule details ›
Chains: A, B
Length: 470 amino acids
Theoretical weight: 51.71 KDa
Source organism: Oryctolagus cuniculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P07511 (Residues: 15-484; Coverage: 97%)
Gene name: SHMT1
Sequence domains: Serine hydroxymethyltransferase
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P41212
Unit cell:
a: 116.38Å b: 116.38Å c: 165.38Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.28
Expression system: Escherichia coli

Quick links

Citations

6 review citations

The manifold of vitamin B6 dependent enzymes.
Schneider et al. (2000)
5 more

3 mentions without citation

Structure-based mechanism for early PLP-mediated steps of rabbit cytosolic serine hydroxymethyltransferase reaction.
Di Salvo et al. (2013)
2 more