› 1cqh

Solution NMR

HIGH RESOLUTION SOLUTION NMR STRUCTURE OF MIXED DISULFIDE INTERMEDIATE BETWEEN HUMAN THIOREDOXIN (C35A, C62A, C69A, C73A) MUTANT AND A 13 RESIDUE PEPTIDE COMPRISING ITS TARGET SITE IN HUMAN REF-1 (RESIDUES 59-71 OF THE P50 SUBUNIT OF NFKB), NMR, MINIMIZED AVERAGE STRUCTURE

Released:
DOI: 10.2210/pdb1cqh/pdb
PDB entry 1cqh coloured by chain, front view.
PDB entry 1cqh coloured by chain, side view.
PDB entry 1cqh coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The solution structure of human thioredoxin complexed with its target from Ref-1 reveals peptide chain reversal.
Qin J, Clore GM, Kennedy WP, Kuszewski J, Gronenborn AM
Structure 4 613-20 (1996)
PMID: 8736558

Function and Biology Details

Reaction catalysed: 
Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145350 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thioredoxin Chain: A
Molecule details ›
Chain: A
Length: 105 amino acids
Theoretical weight: 11.59 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P10599 (Residues: 1-105; Coverage: 100%)
Gene names: TRDX, TRX, TRX1, TXN
Sequence domains: Thioredoxin
Structure domains: Glutaredoxin
DNA repair nuclease/redox regulator APEX1, mitochondrial Chain: B
Molecule details ›
Chain: B
Length: 13 amino acids
Theoretical weight: 1.4 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P27695 (Residues: 59-71; Coverage: 4%)
Gene names: APE, APE1, APEX, APEX1, APX, HAP1, REF1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression system: Not provided

Quick links

Citations

20 review citations

The many functions of APE1/Ref-1: not only a DNA repair enzyme.
Tell et al. (2009)
19 more

4 mentions without citation

Probing the chemistry of thioredoxin catalysis with force.
Wiita et al. (2007)
3 more