› 1d1u

X-ray diffraction
2.3Å resolution

USE OF AN N-TERMINAL FRAGMENT FROM MOLONEY MURINE LEUKEMIA VIRUS REVERSE TRANSCRIPTASE TO FACILITATE CRYSTALLIZATION AND ANALYSIS OF A PSEUDO-16-MER DNA MOLECULE CONTAINING G-A MISPAIRS

Released:
DOI: 10.2210/pdb1d1u/pdb
PDB entry 1d1u coloured by chain, front view.
PDB entry 1d1u coloured by chain, side view.
PDB entry 1d1u coloured by chain, top view.
Source organism: Moloney murine leukemia virus
Primary publication:
Use of an N-terminal fragment from moloney murine leukemia virus reverse transcriptase to facilitate crystallization and analysis of a pseudo-16-mer DNA molecule containing G-A mispairs.
Coté ML, Yohannan SJ, Georgiadis MM
Acta Crystallogr D Biol Crystallogr 56 1120-31 (2000)
PMID: 10957631

Function and Biology Details

Reactions catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113580 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Reverse transcriptase/ribonuclease H Chain: A
Molecule details ›
Chain: A
Length: 255 amino acids
Theoretical weight: 28.93 KDa
Source organism: Moloney murine leukemia virus
Expression system: Escherichia coli
UniProt:
  • Canonical: P03355 (Residues: 683-937; Coverage: 15%)
Gene name: gag-pol
Sequence domains: Reverse transcriptase (RNA-dependent DNA polymerase)
Structure domains:
DNA (5'-D(*CP*TP*CP*GP*TP*G)-3') Chain: B
Molecule details ›
Chain: B
Length: 6 nucleotides
Theoretical weight: 1.8 KDa
Source organism: Moloney murine leukemia virus
Expression system: Not provided
DNA (5'-D(*AP*CP*GP*GP*CP*AP*CP*GP*AP*G)-3') Chain: C
Molecule details ›
Chain: C
Length: 10 nucleotides
Theoretical weight: 3.08 KDa
Source organism: Moloney murine leukemia virus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21212
Unit cell:
a: 54.738Å b: 145.491Å c: 46.737Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.286
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Toward an Expanded Genome: Structural and Computational Characterization of an Artificially Expanded Genetic Information System.
Richards et al. (2017)
16 other articles

2 mentions without citation

PDA: an automatic and comprehensive analysis program for protein-DNA complex structures.
Kim et al. (2009)
1 more