› 1dde

X-ray diffraction
1.7Å resolution

STRUCTURE OF THE DNAG CATALYTIC CORE

Released:
DOI: 10.2210/pdb1dde/pdb
PDB entry 1dde coloured by chain, front view.
PDB entry 1dde coloured by chain, side view.
PDB entry 1dde coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of the RNA polymerase domain of E. coli primase.
Keck JL, Roche DD, Lynch AS, Berger JM
Science 287 2482-6 (2000)
PMID: 10741967

Function and Biology Details

Reaction catalysed: 
SsDNA + n NTP = ssDNA/pppN(pN)(n-1) hybrid + (n-1) diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142202 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA primase Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 38.22 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABS5 (Residues: 111-433; Coverage: 56%)
Gene names: JW3038, b3066, dnaG, dnaP, parB
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand Y1 4 x Y1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 38.02Å b: 55.89Å c: 139.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.263
Expression system: Escherichia coli

Quick links

Citations

21 review citations

SSB as an organizer/mobilizer of genome maintenance complexes.
Shereda et al. (2008)
20 more

18 mentions without citation

Architecture and conservation of the bacterial DNA replication machinery, an underexploited drug target.
Robinson et al. (2012)
17 more