1duz Citations

The structure and stability of an HLA-A*0201/octameric tax peptide complex with an empty conserved peptide-N-terminal binding site.

J Immunol 164 6398-405 (2000)
Cited: 146 times
EuropePMC logo PMID: 10843695

Abstract

The crystal structure of the human class I MHC molecule HLA-A2 complexed with of an octameric peptide, Tax8 (LFGYPVYV), from human T cell lymphotrophic virus-1 (HTLV-1) has been determined. This structure is compared with a newly refined, higher resolution (1.8 A) structure of HLA-A2 complexed with the nonameric Tax9 peptide (LLFGYPVYV) with one more N-terminal residue. Despite the absence of a peptide residue (P1) bound in the conserved N-terminal peptide-binding pocket of the Tax8/HLA-A2 complex, the structures of the two complexes are essentially identical. Water molecules in the Tax8 complex replace the terminal amino group of the Tax9 peptide and mediate a network of hydrogen bonds among the secondary structural elements at that end of the peptide-binding groove. Thermal denaturation measurements indicate that the Tax8 complex is much less stable, DeltaTm = 16 degrees C, than the Tax9 complex, but both can sensitize target cells for lysis by some Tax-specific CTL from HTLV-1 infected individuals. The absence of a P1 peptide residue is thus not enough to prevent formation of a "closed conformation" of the peptide-binding site. TCR affinity measurements and cytotoxic T cell assays indicate that the Tax8/HLA-A2 complex does not functionally cross-react with the A6-TCR-bearing T cell clone specific for Tax9/HLA-A2 complexes.

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  1. T cell antigen receptor recognition of antigen-presenting molecules. Rossjohn J, Gras S, Miles JJ, Turner SJ, Godfrey DI, McCluskey J. Annu Rev Immunol 33 169-200 (2015)
  2. Antigenicity and immunogenicity of Melan-A/MART-1 derived peptides as targets for tumor reactive CTL in human melanoma. Romero P, Valmori D, Pittet MJ, Zippelius A, Rimoldi D, Lévy F, Dutoit V, Ayyoub M, Rubio-Godoy V, Michielin O, Guillaume P, Batard P, Luescher IF, Lejeune F, Liénard D, Rufer N, Dietrich PY, Speiser DE, Cerottini JC. Immunol Rev 188 81-96 (2002)
  3. Conformational changes and flexibility in T-cell receptor recognition of peptide-MHC complexes. Armstrong KM, Piepenbrink KH, Baker BM. Biochem J 415 183-196 (2008)
  4. The optimization of peptide cargo bound to MHC class I molecules by the peptide-loading complex. Elliott T, Williams A. Immunol Rev 207 89-99 (2005)
  5. A structural voyage toward an understanding of the MHC-I-restricted immune response: lessons learned and much to be learned. Gras S, Burrows SR, Turner SJ, Sewell AK, McCluskey J, Rossjohn J. Immunol Rev 250 61-81 (2012)
  6. Understanding the complex mechanisms of β2-microglobulin amyloid assembly. Eichner T, Radford SE. FEBS J 278 3868-3883 (2011)
  7. Tapasin and other chaperones: models of the MHC class I loading complex. Wright CA, Kozik P, Zacharias M, Springer S. Biol Chem 385 763-778 (2004)
  8. Accessory proteins and the assembly of human class I MHC molecules: a molecular and structural perspective. Bouvier M. Mol Immunol 39 697-706 (2003)
  9. Structural studies on HLA-G: implications for ligand and receptor binding. Clements CS, Kjer-Nielsen L, McCluskey J, Rossjohn J. Hum Immunol 68 220-226 (2007)
  10. Virus subversion of immunity: a structural perspective. Gewurz BE, Gaudet R, Tortorella D, Wang EW, Ploegh HL. Curr Opin Immunol 13 442-450 (2001)
  11. Recombinant antibodies with MHC-restricted, peptide-specific, T-cell receptor-like specificity: new tools to study antigen presentation and TCR-peptide-MHC interactions. Cohen CJ, Denkberg G, Lev A, Epel M, Reiter Y. J Mol Recognit 16 324-332 (2003)
  12. beta(2)-microglobulin: from physiology to amyloidosis. Heegaard NH. Amyloid 16 151-173 (2009)
  13. The Impact of the 'Mis-Peptidome' on HLA Class I-Mediated Diseases: Contribution of ERAP1 and ERAP2 and Effects on the Immune Response. Tedeschi V, Paldino G, Paladini F, Mattorre B, Tuosto L, Sorrentino R, Fiorillo MT. Int J Mol Sci 21 E9608 (2020)
  14. T-cell activation by lipopeptide antigens. Van Rhijn I, Zajonc DM, Wilson IA, Moody DB. Curr Opin Immunol 17 222-229 (2005)
  15. Peptide trafficking and translocation across membranes in cellular signaling and self-defense strategies. Abele R, Tampé R. Curr Opin Cell Biol 21 508-515 (2009)
  16. Structural Prediction of Peptide-MHC Binding Modes. Perez MAS, Cuendet MA, Röhrig UF, Michielin O, Zoete V. Methods Mol Biol 2405 245-282 (2022)
  17. "It's Only a Model": When Protein Structure Predictions Need Experimental Validation, the Case of the HTLV-1 Tax Protein. Guillon C, Robert X, Gouet P. Pathogens 13 241 (2024)

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