› 1dvf

X-ray diffraction
1.9Å resolution

IDIOTOPIC ANTIBODY D1.3 FV FRAGMENT-ANTIIDIOTOPIC ANTIBODY E5.2 FV FRAGMENT COMPLEX

Released:
DOI: 10.2210/pdb1dvf/pdb
PDB entry 1dvf coloured by chain, front view.
PDB entry 1dvf coloured by chain, side view.
PDB entry 1dvf coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of an Fv-Fv idiotope-anti-idiotope complex at 1.9 A resolution.
Braden BC, Fields BA, Ysern X, Dall'Acqua W, Goldbaum FA, Poljak RJ, Mariuzza RA
J Mol Biol 264 137-51 (1996)
PMID: 8950273

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero tetramer (preferred)
hetero octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-499112 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Immunoglobulin kappa chain variable 12-41 Chain: A
Molecule details ›
Chain: A
Length: 108 amino acids
Theoretical weight: 11.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01635 (Residues: 21-115; Coverage: 100%)
Gene names: Gm16848, Igkv12-41
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig heavy chain V region PJ14 Chain: B
Molecule details ›
Chain: B
Length: 116 amino acids
Theoretical weight: 12.86 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01820 (Residues: 20-115; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig kappa chain V-V region HP 123E6 Chain: C
Molecule details ›
Chain: C
Length: 107 amino acids
Theoretical weight: 11.77 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P01646 (Residues: 1-107; Coverage: 99%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: D
Molecule details ›
Chain: D
Length: 121 amino acids
Theoretical weight: 13.32 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: A0NA69 (Residues: 20-117; Coverage: 95%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: C2
Unit cell:
a: 152.8Å b: 79.4Å c: 51.5Å
α: 90° β: 100.2° γ: 90°
R-values:
R R work R free
0.194 0.194 0.249
Expression system: Escherichia coli

Quick links

Citations

5 review citations

B cell epitope spreading: mechanisms and contribution to autoimmune diseases.
Cornaby et al. (2015)
4 more

27 mentions without citation

Analysis and Ranking of Protein-Protein Docking Models Using Inter-Residue Contacts and Inter-Molecular Contact Maps.
Oliva et al. (2015)
26 more