› 1e30

X-ray diffraction
1.5Å resolution

Crystal structure of the Met148Gln mutant of rusticyanin at 1.5 Angstrom resolution

Released:
DOI: 10.2210/pdb1e30/pdb
PDB entry 1e30 coloured by chain, front view.
PDB entry 1e30 coloured by chain, side view.
PDB entry 1e30 coloured by chain, top view.
Source organism: Acidithiobacillus ferrooxidans
Primary publication:
Role of the axial ligand in type 1 Cu centers studied by point mutations of met148 in rusticyanin.
Hall JF, Kanbi LD, Strange RW, Hasnain SS
Biochemistry 38 12675-80 (1999)
PMID: 10504237

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143185 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rusticyanin Chains: A, B
Molecule details ›
Chains: A, B
Length: 155 amino acids
Theoretical weight: 16.57 KDa
Source organism: Acidithiobacillus ferrooxidans
Expression system: Escherichia coli
UniProt:
  • Canonical: P0C918 (Residues: 33-187; Coverage: 100%)
Gene names: rus, rusTA
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 2 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P21
Unit cell:
a: 42.861Å b: 61.38Å c: 53.33Å
α: 90° β: 96.19° γ: 90°
R-values:
R R work R free
0.182 0.182 0.217
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Metalloproteins containing cytochrome, iron-sulfur, or copper redox centers.
Liu et al. (2014)
9 more

2 mentions without citation

Homeodomain proteins: an update.
Bürglin et al. (2016)
1 more