› 1e5f

X-ray diffraction
2.18Å resolution

METHIONINE GAMMA-LYASE (MGL) FROM TRICHOMONAS VAGINALIS

Released:
DOI: 10.2210/pdb1e5f/pdb
PDB entry 1e5f coloured by chain, front view.
PDB entry 1e5f coloured by chain, side view.
PDB entry 1e5f coloured by chain, top view.
Source organism: Trichomonas vaginalis G3
Entry authors: Goodall G, Mottram JC, Coombs GH, Lapthorn AJ

Function and Biology Details

Reaction catalysed: 
(1a) L-methionine = methanethiol + 2-aminobut-2-enoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127536 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-methionine gamma-lyase Chains: A, B
Molecule details ›
Chains: A, B
Length: 404 amino acids
Theoretical weight: 44.15 KDa
Source organism: Trichomonas vaginalis G3
Expression system: Escherichia coli
UniProt:
  • Canonical: O15564 (Residues: 1-396; Coverage: 100%)
Gene name: mgl1
Sequence domains: Cys/Met metabolism PLP-dependent enzyme
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 2 x PLP
2 bound ligands:
Ligand SO4 6 x SO4
Ligand GOL 1 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.5
Spacegroup: P3112
Unit cell:
a: 88.258Å b: 88.258Å c: 217.849Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.162 0.162 0.212
Expression system: Escherichia coli

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