› 1e6q

X-ray diffraction
1.35Å resolution

MYROSINASE FROM SINAPIS ALBA with the bound transition state analogue gluco-tetrazole

Released:
DOI: 10.2210/pdb1e6q/pdb
PDB entry 1e6q coloured by chain, front view.
PDB entry 1e6q coloured by chain, side view.
PDB entry 1e6q coloured by chain, top view.
Source organism: Sinapis alba
Primary publication:
High resolution X-ray crystallography shows that ascorbate is a cofactor for myrosinase and substitutes for the function of the catalytic base.
Burmeister WP, Cottaz S, Rollin P, Vasella A, Henrissat B
J Biol Chem 275 39385-93 (2000)
PMID: 10978344

Function and Biology Details

Reaction catalysed: 
A thioglucoside + H(2)O = a sugar + a thiol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151569 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (4 distinct):
Myrosinase MA1 Chain: M
Molecule details ›
Chain: M
Length: 501 amino acids
Theoretical weight: 57.08 KDa
Source organism: Sinapis alba
UniProt:
  • Canonical: P29736 (Residues: 1-501; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 1
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, XYP, FUC
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG, BMA, XYP, FUC, MAN
Carbohydrate polymer
5 bound ligands:
Ligand NAG 6 x NAG
Ligand NTZ 1 x NTZ
Ligand GOL 4 x GOL
Ligand SO4 7 x SO4
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2221
Unit cell:
a: 135.3Å b: 137.2Å c: 80.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 0.119 0.146

Quick links

Citations

22 review citations

The chemical diversity and distribution of glucosinolates and isothiocyanates among plants.
Fahey et al. (2001)
21 more

1 mention without citation

Fragmentation-tree density representation for crystallographic modelling of bound ligands.
Langer et al. (2012)