› 1edq

X-ray diffraction
1.55Å resolution

CRYSTAL STRUCTURE OF CHITINASE A FROM S. MARCESCENS AT 1.55 ANGSTROMS

Released:
DOI: 10.2210/pdb1edq/pdb
PDB entry 1edq coloured by chain, front view.
PDB entry 1edq coloured by chain, side view.
PDB entry 1edq coloured by chain, top view.
Source organism: Serratia marcescens
Primary publication:
De novo purification scheme and crystallization conditions yield high-resolution structures of chitinase A and its complex with the inhibitor allosamidin.
Papanikolau Y, Tavlas G, Vorgias CE, Petratos K
Acta Crystallogr D Biol Crystallogr 59 400-3 (2003)
PMID: 12554965

Function and Biology Details

Reaction catalysed: 
Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-131383 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
GH18 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 540 amino acids
Theoretical weight: 58.64 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
UniProt:
  • Canonical: O83008 (Residues: 24-563; Coverage: 100%)
Gene name: chiA
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: C2221
Unit cell:
a: 199.497Å b: 131.825Å c: 59.368Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.215
Expression system: Escherichia coli

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Citations

3 review citations

Natural product family 18 chitinase inhibitors.
Andersen et al. (2005)
2 more

12 mentions without citation

Chitinase: diversity, limitations, and trends in engineering for suitable applications.
Oyeleye et al. (2018)
11 more