› 1ef0

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF PI-SCEI MINIPRECURSOR

Released:
DOI: 10.2210/pdb1ef0/pdb
Model geometry
Fit model/data
PDB entry 1ef0 coloured by chain, front view.
PDB entry 1ef0 coloured by chain, side view.
PDB entry 1ef0 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural insights into the protein splicing mechanism of PI-SceI.
Poland BW, Xu MQ, Quiocho FA
J Biol Chem 275 16408-13 (2000)
PMID: 10828056

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147905 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endonuclease PI-SceI Chains: A, B
Molecule details ›
Chains: A, B
Length: 462 amino acids
Theoretical weight: 51.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P17255 (Residues: 283-741; Coverage: 43%)
Gene names: CLS8, D1286, TFP1, VMA1, YDL185W
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 59.003Å b: 101.683Å c: 86.765Å
α: 90° β: 93.51° γ: 90°
R-values:
R R work R free
0.231 0.231 0.281
Expression system: Escherichia coli

Quick links

Citations

16 review citations

Inteins: structure, function, and evolution.
Gogarten et al. (2002)
15 more

6 mentions without citation

Crystallographic and mutational studies of Mycobacterium tuberculosis recA mini-inteins suggest a pivotal role for a highly conserved aspartate residue.
Van Roey et al. (2007)
5 more