› 1egy

X-ray diffraction
2.35Å resolution

CYTOCHROME P450ERYF WITH 9-AMINOPHENANTHRENE BOUND

Released:
DOI: 10.2210/pdb1egy/pdb
PDB entry 1egy coloured by chain, front view.
PDB entry 1egy coloured by chain, side view.
PDB entry 1egy coloured by chain, top view.
Source organism: Saccharopolyspora erythraea
Primary publication:
Crystal structures of ligand complexes of P450eryF exhibiting homotropic cooperativity.
Cupp-Vickery J, Anderson R, Hatziris Z
Proc Natl Acad Sci U S A 97 3050-5 (2000)
PMID: 10716705

Function and Biology Details

Reaction catalysed: 
6-deoxyerythronolide B + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = erythronolide B + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-169415 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
6-deoxyerythronolide B hydroxylase Chain: A
Molecule details ›
Chain: A
Length: 403 amino acids
Theoretical weight: 44.52 KDa
Source organism: Saccharopolyspora erythraea
UniProt:
  • Canonical: Q00441 (Residues: 2-404; Coverage: 100%)
Gene names: CYP107A1, SACE_0730, eryF
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

2 bound ligands:
Ligand HEM 1 x HEM
Ligand 9AP 2 x 9AP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P212121
Unit cell:
a: 54.001Å b: 78.429Å c: 98.868Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 not available not available

Quick links

Citations

19 review citations

Multiple molecular mechanisms for multidrug resistance transporters.
Higgins CF. (2007)
18 more

7 mentions without citation

Conformational plasticity and structure/function relationships in cytochromes P450.
Pochapsky et al. (2010)
6 more