› 1ei5

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF A D-AMINOPEPTIDASE FROM OCHROBACTRUM ANTHROPI

Released:
DOI: 10.2210/pdb1ei5/pdb
PDB entry 1ei5 coloured by chain, front view.
PDB entry 1ei5 coloured by chain, side view.
PDB entry 1ei5 coloured by chain, top view.
Source organism: Brucella anthropi
Primary publication:
Crystal structure of a D-aminopeptidase from Ochrobactrum anthropi, a new member of the 'penicillin-recognizing enzyme' family.
Bompard-Gilles C, Remaut H, Villeret V, Prangé T, Fanuel L, Delmarcelle M, Joris B, Frère J, Van Beeumen J
Structure 8 971-80 (2000)
PMID: 10986464

Function and Biology Details

Reaction catalysed: 
Release of an N-terminal D-amino acid from a peptide, Xaa-|-Yaa-, in which Xaa is preferably D-Ala, D-Ser or D-Thr. D-amino acid amides and methyl esters also are hydrolyzed, as is glycine amide.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195609 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-aminopeptidase Chain: A
Molecule details ›
Chain: A
Length: 520 amino acids
Theoretical weight: 57.46 KDa
Source organism: Brucella anthropi
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZBA9 (Residues: 1-520; Coverage: 100%)
Gene name: dap
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LURE BEAMLINE DW32
Spacegroup: P41212
Unit cell:
a: 82.86Å b: 82.86Å c: 204.65Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.163 0.192
Expression system: Escherichia coli

Quick links

Citations

2 review citations

The penicillin-binding proteins: structure and role in peptidoglycan biosynthesis.
Sauvage et al. (2008)
1 more

12 mentions without citation

Staphostatins resemble lipocalins, not cystatins in fold.
Rzychon et al. (2003)
11 more