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Direct structural observation of an acyl-enzyme intermediate in the hydrolysis of an ester substrate by elastase.

Ding X, Rasmussen BF, Petsko GA, Ringe D
Biochemistry 33 9285-93 (1994)
Cited: 41 times
EuropePMC logo PMID: 8049229

Abstract

The method of X-ray crystallographic cryoenzymology has been used to determine the crystal structure of a kinetically significant species on the reaction pathway of a crystalline enzyme. The structure of a specific acyl-enzyme intermediate in the elastase-catalyzed hydrolysis of the N-carbobenzoxy-L-alanine p-nitrophenyl ester has been determined and refined against X-ray diffraction data at 2.3-A resolution. The difference Fourier electron density map clearly shows electron density for the trapped acyl-enzyme. The acyl-enzyme was formed at -26 degrees C and was stabilized at -55 degrees C during data collection, taking advantage of the glass transition in protein dynamics that occurs at around -50 degrees C.

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Related citations provided by authors (1)

  1. Structure of Native Procine Pancreatic Elastase at 1.65 Angstroms Resolution. Meyer E, Cole G, Radhakrishnan R, Epp O Acta Crystallogr., B 44 26- (1988)

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