› 1eue

X-ray diffraction
1.8Å resolution

RAT OUTER MITOCHONDRIAL MEMBRANE CYTOCHROME B5

Released:
DOI: 10.2210/pdb1eue/pdb
PDB entry 1eue coloured by chain, front view.
PDB entry 1eue coloured by chain, side view.
PDB entry 1eue coloured by chain, top view.
Source organism: Rattus norvegicus
Primary publication:
Modulation of redox potential in electron transfer proteins: effects of complex formation on the active site microenvironment of cytochrome b5.
Wirtz M, Oganesyan V, Zhang X, Studer J, Rivera M
Faraday Discuss 221-34; discussion 257-68 (2000)
PMID: 11197480

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-137474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 type B Chains: A, B
Molecule details ›
Chains: A, B
Length: 86 amino acids
Theoretical weight: 9.85 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P04166 (Residues: 17-102; Coverage: 59%)
Gene names: Cyb5b, Cyb5m, Omb5
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

1 bound ligand:
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 46.23Å b: 70.77Å c: 72.44Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.197 0.232
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Redox and redox-coupled processes of heme proteins and enzymes at electrochemical interfaces.
Murgida et al. (2005)
9 other articles

2 mentions without citation

Study of the individual cytochrome b5 and cytochrome b5 reductase domains of Ncb5or reveals a unique heme pocket and a possible role of the CS domain.
Deng et al. (2010)
1 more