› 1eui

X-ray diffraction
3.2Å resolution

ESCHERICHIA COLI URACIL-DNA GLYCOSYLASE COMPLEX WITH URACIL-DNA GLYCOSYLASE INHIBITOR PROTEIN

Released:
DOI: 10.2210/pdb1eui/pdb
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PDB entry 1eui coloured by chain, front view.
PDB entry 1eui coloured by chain, side view.
PDB entry 1eui coloured by chain, top view.
Source organisms:
Primary publication:
X-ray analysis of a complex of Escherichia coli uracil DNA glycosylase (EcUDG) with a proteinaceous inhibitor. The structure elucidation of a prokaryotic UDG.
Ravishankar R, Bidya Sagar M, Roy S, Purnapatre K, Handa P, Varshney U, Vijayan M
Nucleic Acids Res 26 4880-7 (1998)
PMID: 9776748

Function and Biology Details

Reaction catalysed: 
Hydrolyzes single-stranded DNA or mismatched double-stranded DNA and polynucleotides, releasing free uracil
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146130 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Uracil-DNA glycosylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 228 amino acids
Theoretical weight: 25.59 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P12295 (Residues: 2-229; Coverage: 100%)
Gene names: JW2564, b2580, ung
Sequence domains: Uracil DNA glycosylase superfamily
Structure domains: Uracil-DNA glycosylase-like domain
Uracil-DNA glycosylase inhibitor Chains: C, D
Molecule details ›
Chains: C, D
Length: 84 amino acids
Theoretical weight: 9.48 KDa
Source organism: Bacillus phage PBS2
UniProt:
  • Canonical: P14739 (Residues: 1-84; Coverage: 100%)
Gene name: UGI
Sequence domains: Uracil-DNA glycosylase inhibitor
Structure domains: Bacteriophage PBS2, uracil-glycosylase inhibitor

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

wwPDB Validation report is not available for this entry.
X-ray source: RIGAKU RUH2R
Spacegroup: P212121
Unit cell:
a: 51.373Å b: 89.772Å c: 142.132Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.274

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Citations

1 review citation

Uracil-DNA glycosylase: Structural, thermodynamic and kinetic aspects of lesion search and recognition.
Zharkov et al. (2010)
29 other articles

6 mentions without citation

Uracil-DNA glycosylases-structural and functional perspectives on an essential family of DNA repair enzymes.
Schormann et al. (2014)
5 more

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