› 1f4m

X-ray diffraction
2.25Å resolution

P3(2) CRYSTAL STRUCTURE OF ALA2ILE2-6, A VERSION OF ROP WITH A REPACKED HYDROPHOBIC CORE AND A NEW FOLD.

Released:
DOI: 10.2210/pdb1f4m/pdb
PDB entry 1f4m coloured by chain, front view.
PDB entry 1f4m coloured by chain, side view.
PDB entry 1f4m coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Dramatic structural and thermodynamic consequences of repacking a protein's hydrophobic core.
Willis MA, Bishop B, Regan L, Brunger AT
Structure 8 1319-28 (2000)
PMID: 11188696

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Regulatory protein rop Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 63 amino acids
Theoretical weight: 7.05 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P03051 (Residues: 1-63; Coverage: 100%)
Gene name: rop
Sequence domains: Rop protein
Structure domains: Helix Hairpins

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P32
Unit cell:
a: 73.092Å b: 73.092Å c: 65.921Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.233 0.233 0.282
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Rotamer libraries in the 21st century.
Dunbrack RL. (2002)
3 more

4 mentions without citation

Structural space of protein-protein interfaces is degenerate, close to complete, and highly connected.
Gao et al. (2010)
3 more