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Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity.

Villeneuve S, Souchon H, Riottot MM, Mazie JC, Lei P, Glaudemans CP, Kovác P, Fournier JM, Alzari PM
Proc Natl Acad Sci U S A 97 8433-8 (2000)
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Cited: 78 times
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Abstract

The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is shown to be the primary antigenic determinant. Additional perosamine residues protrude outwards from the Ab surface and contribute only marginally to the binding affinity and specificity. A complementary water-excluding hydrophobic interface and five Ab-Ag hydrogen bonds are crucial for carbohydrate recognition. The structure reported here explains the serotype specificity of anti-Ogawa Abs and provides a rational basis toward the development of a synthetic carbohydrate-based anti-cholera vaccine.

Articles - 1f4y mentioned but not cited (2)

  1. Crystal structure of an anti-carbohydrate antibody directed against Vibrio cholerae O1 in complex with antigen: molecular basis for serotype specificity. Villeneuve S, Souchon H, Riottot MM, Mazie JC, Lei P, Glaudemans CP, Kovác P, Fournier JM, Alzari PM. Proc Natl Acad Sci U S A 97 8433-8438 (2000)
  2. Generation and Characterization of Monoclonal Antibodies to the Ogawa Lipopolysaccharide of Vibrio cholerae O1 from Phage-Displayed Human Synthetic Fab Library. Kim D, Hong J, Choi Y, Han J, Kim S, Jo G, Yoon JY, Chae H, Yoon H, Lee C, Hong HJ. J Microbiol Biotechnol 30 1760-1768 (2020)


Reviews citing this publication (10)

  1. Antibody recognition of carbohydrate epitopes†. Haji-Ghassemi O, Blackler RJ, Martin Young N, Evans SV. Glycobiology 25 920-952 (2015)
  2. Chemical biology approaches to designing defined carbohydrate vaccines. Anish C, Schumann B, Pereira CL, Seeberger PH. Chem Biol 21 38-50 (2014)
  3. Antimicrobial glycoconjugate vaccines: an overview of classic and modern approaches for protein modification. Berti F, Adamo R. Chem Soc Rev 47 9015-9025 (2018)
  4. PaxVax CVD 103-HgR single-dose live oral cholera vaccine. Levine MM, Chen WH, Kaper JB, Lock M, Danzig L, Gurwith M. Expert Rev Vaccines 16 197-213 (2017)
  5. Designing a new antifungal glycoconjugate vaccine. Johnson MA, Bundle DR. Chem Soc Rev 42 4327-4344 (2013)
  6. Vibrio cholerae: lessons for mucosal vaccine design. Bishop AL, Camilli A. Expert Rev Vaccines 10 79-94 (2011)
  7. The ABCs (Antibody, B cells, and Carbohydrate epitopes) of cholera immunity: considerations for an improved vaccine. Provenzano D, Kovác P, Wade WF. Microbiol Immunol 50 899-927 (2006)
  8. Lipopolysaccharides of Vibrio cholerae: III. Biological functions. Chatterjee SN, Chaudhuri K. Biochim Biophys Acta 1762 1-16 (2006)
  9. Development in the Concept of Bacterial Polysaccharide Repeating Unit-Based Antibacterial Conjugate Vaccines. Rohokale R, Guo Z. ACS Infect Dis 9 178-212 (2023)
  10. [Carbohydrates and vaccines: from purified polysaccharides to semi-synthetic glycoconjugate vaccines]. Mulard L. Ann Pharm Fr 65 14-32 (2007)

Articles citing this publication (66)



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