› 1f5m

X-ray diffraction
1.9Å resolution

STRUCTURE OF THE GAF DOMAIN

Released:
DOI: 10.2210/pdb1f5m/pdb
PDB entry 1f5m coloured by chain, front view.
PDB entry 1f5m coloured by chain, side view.
PDB entry 1f5m coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of the GAF domain, a ubiquitous signaling motif and a new class of cyclic GMP receptor.
Ho YS, Burden LM, Hurley JH
EMBO J 19 5288-99 (2000)
PMID: 11032796

Function and Biology Details

Reaction catalysed: 
L-methionine + thioredoxin disulfide + H(2)O = L-methionine (R)-S-oxide + thioredoxin
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-153114 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Free methionine-R-sulfoxide reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 180 amino acids
Theoretical weight: 19.75 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P36088 (Residues: 1-180; Coverage: 100%)
Gene names: YKL069W, YKL340
Sequence domains: GAF domain
Structure domains: Beta-Lactamase

Ligands and Environments

1 bound ligand:
Ligand BR 9 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P43212
Unit cell:
a: 73.64Å b: 73.64Å c: 162.48Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.214 0.246
Expression system: Escherichia coli

Quick links

Citations

53 review citations

Cyclic di-GMP: the first 25 years of a universal bacterial second messenger.
Römling et al. (2013)
52 more

11 mentions without citation

Bacterial signal transduction network in a genomic perspective.
Galperin MY. (2004)
10 more