› 1fan

X-ray diffraction
2Å resolution

CREVICE-FORMING MUTANTS IN THE RIGID CORE OF BOVINE PANCREATIC TRYPSIN INHIBITOR: CRYSTAL STRUCTURES OF F22A, Y23A, N43G, AND F45A

Released:
DOI: 10.2210/pdb1fan/pdb
PDB entry 1fan coloured by chain, front view.
PDB entry 1fan coloured by chain, side view.
PDB entry 1fan coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Crevice-forming mutants in the rigid core of bovine pancreatic trypsin inhibitor: crystal structures of F22A, Y23A, N43G, and F45A.
Danishefsky AT, Housset D, Kim KS, Tao F, Fuchs J, Woodward C, Wlodawer A
Protein Sci 2 577-87 (1993)
PMID: 8518731

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133749 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pancreatic trypsin inhibitor Chain: A
Molecule details ›
Chain: A
Length: 58 amino acids
Theoretical weight: 6.45 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P00974 (Residues: 36-93; Coverage: 73%)
Sequence domains: Kunitz/Bovine pancreatic trypsin inhibitor domain
Structure domains: Pancreatic trypsin inhibitor Kunitz domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 69.31Å b: 23.19Å c: 29.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.169 0.169 not available
Expression system: Not provided

Quick links

Citations

1 review citation

Core-packing constraints, hydrophobicity and protein design.
Baldwin et al. (1994)
16 other articles

2 mentions without citation

Thoroughly sampling sequence space: large-scale protein design of structural ensembles.
Larson et al. (2002)
1 more