› 1fbl

X-ray diffraction
2.5Å resolution

STRUCTURE OF FULL-LENGTH PORCINE SYNOVIAL COLLAGENASE (MMP1) REVEALS A C-TERMINAL DOMAIN CONTAINING A CALCIUM-LINKED, FOUR-BLADED BETA-PROPELLER

Released:
DOI: 10.2210/pdb1fbl/pdb
PDB entry 1fbl coloured by chain, front view.
PDB entry 1fbl coloured by chain, side view.
PDB entry 1fbl coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Structure of full-length porcine synovial collagenase reveals a C-terminal domain containing a calcium-linked, four-bladed beta-propeller.
Li J, Brick P, O'Hare MC, Skarzynski T, Lloyd LF, Curry VA, Clark IM, Bigg HF, Hazleman BL, Cawston TE
Structure 3 541-9 (1995)
PMID: 8590015

Function and Biology Details

Reaction catalysed: 
Cleavage of the triple helix of collagen at about three-quarters of the length of the molecule from the N-terminus, at 775-Gly-|-Ile-776 in the alpha-1(I) chain. Cleaves synthetic substrates and alpha-macroglobulins at bonds where P1' is a hydrophobic residue.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149362 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interstitial collagenase Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 42.69 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P21692 (Residues: 100-469; Coverage: 82%)
Gene name: MMP1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand CA 4 x CA
Ligand ZN 2 x ZN
Ligand HTA 1 x HTA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: I41
Unit cell:
a: 161.14Å b: 161.14Å c: 52.22Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.217 not available
Expression system: Escherichia coli

Quick links

Citations

44 review citations

Matrix metalloproteinases and tissue inhibitors of metalloproteinases: structure, function, and biochemistry.
Visse et al. (2003)
43 more

16 mentions without citation

Microfibrillar structure of type I collagen in situ.
Orgel et al. (2006)
15 more