› 1fio

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF YEAST T-SNARE PROTEIN SSO1

Released:
DOI: 10.2210/pdb1fio/pdb
PDB entry 1fio coloured by chain, front view.
PDB entry 1fio coloured by chain, side view.
PDB entry 1fio coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Interactions within the yeast t-SNARE Sso1p that control SNARE complex assembly.
Munson M, Chen X, Cocina AE, Schultz SM, Hughson FM
Nat Struct Biol 7 894-902 (2000)
PMID: 11017200

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152485 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein SSO1 Chain: A
Molecule details ›
Chain: A
Length: 196 amino acids
Theoretical weight: 22.8 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P32867 (Residues: 31-225; Coverage: 67%)
Gene names: P1405, SSO1, YPL232W
Sequence domains: Syntaxin
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 42.517Å b: 49.855Å c: 113.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.219 0.265
Expression system: Escherichia coli

Quick links

Citations

25 review citations

SNAREs--engines for membrane fusion.
Jahn et al. (2006)
24 more

8 mentions without citation

Sec3 promotes the initial binary t-SNARE complex assembly and membrane fusion.
Yue et al. (2017)
7 more